蔡希陶论坛第36期

Title: Dissecting the Regulatory Genome: Making Sense from Nonsense

题目：调控基因组解析，揭示非编码区的奥秘 Speaker: Prof. Michael Q. Zhang Cecil H. and Ida Green Distinguished Chair Professor, Director of Center for Systems Biology, UT Dallas. USA Qian-Ren Guest Professor, Director of Center for Synthetic & Systems Biology, Tsinghua University. China

主 持 人：刘长宁 研究员（中国科学院西双版纳热带植物园）

时 间：2019年8月2日(星期五)上午 10:00-12:00

地 点：园部蔡希陶会议室及昆明分部101会议室（视频）

Time: 10:00 AM, August 2, 2019；

Venue: The Cai Xitao meeting room in Xishuangbanna Headquarter and The 101 meeting room in Kunming Division (video conference)

Abstract: The human genome contains ~20,000 genes, but majority of the genome is noncoding. Dissecting cis-regulatory elements hidden in these regions and understanding of the mechanisms by which they exert regulatory effects on specific target genes across distances of kilobases, and in some cases megabases, remain very challenging. I will briefly review our technology development in studying cis-regulatory structures and functional genomics over the years. Then introduce our recent developments in biochemical (BL-Hi-C), biophysical (Super-resolution imaging) and Bioinformatics (Functional DNA loop quantitation and prediction) technologies, in particular on a new methodology CAPTURE -- “In Situ Capture of Chromatin Interactions by Biotinylated dCas9” and its application for in situ capture of individual constituents of the enhancer cluster controlling human β-globin genes, which establishes evidence for composition-based hierarchical 3D regulatory organization and its implication in human diseases.